apoptosis assay on adherent cells

From: Anke Kretz (kretz-rommel@alxnsd.com)
Date: Fri May 18 2001 - 15:15:41 EST 

I am trying to determine apoptosis induction on adherent tumor cells.  I
used annexin/PI staining, however the background of annexin staining in
untreated cells was up to 20 %.  I am concerned that the cell
dissociation buffer already induces apoptosis.  I also tried IHC, but
was loosing a lot of cells when I was trying to fix them.  Does anybody
have a good protocol how to do apoptosis measurements in adherent cells?

thank you,
Anke

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