An alternative indicator, zinquin, has been used extensively. An overview is at: http://www.chem.northwestern.edu/~ohallo/zinc.html I've used in flow excited by uv from an argon laser; it works well. Apparently, cadmium is the only competitor for binding. Dave dcoder1@qwest.net ----- Original Message ----- From: Kathleen Schell <kschell@facstaff.wisc.edu> To: cyto-inbox Sent: Thursday, May 17, 2001 10:39 AM > > I have an investigator who wants to measure levels of Zn++ in his > cell lines. Has anyone had any experience with Fura-2 that could > give us some pointers? We are planning to excite this dye with a > multi-line UV and collect the emission at 510. The literature that > we have found just uses spectrophotometry to measure this metal. Can > we do this? > Thanks, > Kathy > -- > -------------------------------------------------------------- > Kathy Schell > Supervisor, UWCCC Flow Cytometry Facility > 600 Highland Ave. K4/535 > Madison, WI 53792 > Voice: 608-263-0313 > e-mail: kschell@facstaff.wisc.edu >
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