I think we may be getting several separate issues merged together....
They might be different manifestations of the same underlying cause,
but I suspect not.
There is the general high background that is probably Fc related.
There is also a very specific effect where Cy5 binds to cells of
certain mouse strains; I know it happens with NOD mice, and at
least one other strain that I can't remember.
The mention of CD64 binding in the Blood article is new to me, I'll
have to follow that up and check if it is related to our problem
with Cy5 in NOD mice.
At 08:58 19/4/2001, Mark Kukuruga wrote:
>All,
>I don't recall specifically (hopefully, others will), but I don't
>think it's an Fc problem but rather something about the Cy5 . . .
{snippity snippity ... OUCH!}
--
Joseph Webster, Flow Cytometry Facility
Centenary Institute, Sydney AUSTRALIA.
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