One comment about the suggestion of gating on small cells in order to improve the histogram profiles of cells stained with CFSE. You have to be aware of the fact that cells that are in the process of proliferating are large. If you gate on the small cells, you will exclude the cells that have lower CFSE intensity because they have divided. Alice Alice L. Givan Englert Cell Analysis Laboratory of the Norris Cotton Cancer Center Dartmouth Medical School Lebanon, New Hampshire NH 03756 tel 603-650-7661 fax 603-650-6130 givan@dartmouth.edu
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:01:15 EST