Tom Fleisher and Jerry Marti are experts on this. Perhaps they will comment. Maryalice >Dear community, >some (long) time ago, Maryalice gave the following short answer to a >question posted >by Kathleen. > > >Lithium heparin is toxic to lymphoid cells. We avoid it. > >Since Lithium heparin Vacutainers are for whatever reason a very >common thing in >hospitals this topic is worth being discussed. >The preparation of lymphoid cells from peripheral blood has other >pitfalls, too, >but this is one that could be avoided very easily. >We usually use Na- heparin for research purposes, but sometimes we >get blood samples >with Li- heparin from the clinic. > >Chosing the right blood collection tube for a complicated >application often needs a >lot of experience. >Could someone put a little more light into that matter, I appreciate >any input. >Is there any extensive synopsis about it? > > >Yours, Bernhard > > > >Lithium heparin is toxic to lymphoid cells. We avoid it. > > > > Maryalice > > > > > >>Does anyone have any experience with having used whole blood collected in > >>lithium heparin and then following a staining protocol for surface markers > >>as well as intracellular markers? Thanks in advance. > >> > >> > >>Kathleen MacColl > >>UC Davis Medical Center > > > >Maryalice Stetler-Stevenson > >Director Flow Cytometry Unit > >Laboratory of Pathology, NCI, NIH > >Maryalice Stetler-Stevenson <stetler@box-s.nih.gov> > > Maryalice Stetler-Stevenson Director Flow Cytometry Unit Laboratory of Pathology, NCI, NIH "Learn the rules so you know how to break them properly." The Dalai Lama
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:01:14 EST