Dear community, some (long) time ago, Maryalice gave the following short answer to a question posted by Kathleen. >Lithium heparin is toxic to lymphoid cells. We avoid it. Since Lithium heparin Vacutainers are for whatever reason a very common thing in hospitals this topic is worth being discussed. The preparation of lymphoid cells from peripheral blood has other pitfalls, too, but this is one that could be avoided very easily. We usually use Na- heparin for research purposes, but sometimes we get blood samples with Li- heparin from the clinic. Chosing the right blood collection tube for a complicated application often needs a lot of experience. Could someone put a little more light into that matter, I appreciate any input. Is there any extensive synopsis about it? Yours, Bernhard >Lithium heparin is toxic to lymphoid cells. We avoid it. > > Maryalice > > >>Does anyone have any experience with having used whole blood collected in >>lithium heparin and then following a staining protocol for surface markers >>as well as intracellular markers? Thanks in advance. >> >> >>Kathleen MacColl >>UC Davis Medical Center > >Maryalice Stetler-Stevenson >Director Flow Cytometry Unit >Laboratory of Pathology, NCI, NIH >Maryalice Stetler-Stevenson <stetler@box-s.nih.gov> >
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