Muhammad, Which rhodamine? If you're using the scope excitation at 514 (bright green) then your rhodamine may give a good signal by microscopy, yet not fluoresce well (or at all) excited with the 488 nm line from your laser. If you can tune your laser to 514 nm, try that. If you have a fixed output laser (most analyzers do), then you need a different dye. MAK. -- Mark A. KuKuruga, Managing Director University of Michigan Flow Core 7416 CCGC 0946 (734) 647-3216, fax (734) 936-7376 kukuru@umich.edu >>> Muhammad Anzar <manzar@uoguelph.ca> 03/28/01 02:25PM >>> Hello Friends: We are working on the uptake of a rhodamine-labeled protein by spermatozoa. Under fluorescent microscope, there is clear fluorescence on sperm head but but it can not be detected by flow cytometer (argon ion laser of 488 nm) using FL3 (wavelength 580). Two different flow cytometers have been tried but the results are same. Your suggestions in solving this problem would be highly appreciated. Muhammad Anzar DVM, PhD Department of Animal and Poultry Science University of Guelph Guelph, Ontario, Canada N1G 2W1 Phone: 519-824-4120 (x3689 Off; x8355 Lab) Fax: 519-836-98733 -- ÐÏࡱá
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