Hello Friends: We are working on the uptake of a rhodamine-labeled protein by spermatozoa. Under fluorescent microscope, there is clear fluorescence on sperm head but but it can not be detected by flow cytometer (argon ion laser of 488 nm) using FL3 (wavelength 580). Two different flow cytometers have been tried but the results are same. Your suggestions in solving this problem would be highly appreciated. Muhammad Anzar DVM, PhD Department of Animal and Poultry Science University of Guelph Guelph, Ontario, Canada N1G 2W1 Phone: 519-824-4120 (x3689 Off; x8355 Lab) Fax: 519-836-98733 -- ÐÏࡱá
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