GFP in bacteria

From: Elena Soriano (E.Soriano@iam.boku.ac.at)
Date: Thu Mar 29 2001 - 07:59:05 EST

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I´m trying to measure the GFP expression in bacteria, and I´ve got a nice
signal, but not for all samples, though they have all been treated the
same. We fixed them with 4% formaldehid in PBS at 4°C.
I´ve been told that the GFP is quite small, could it be that it gets
through the fixed cell wall?
Could somebody give me an advice? Does it exist a better kind for fixing
the bacteria for GFP analysis?

Thanks in advance!!



-----------------------------------------
Elena Soriano
c/o Institut für Angewandte Mikrobiologie
    Univ.f.Bodenkultur Wien

Tel: +43 1 36006 6241
Fax: +43 1 36 97 615
http://www.boku.ac.at/iam

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