Apoptosis, Annexin V and Ficoll

From: PLopez@adarc.org
Date: Wed Mar 21 2001 - 17:02:48 EST 

Hello,

I have two questions related to the Annexin V apoptosis thread:

1- In human PBMC preps, would the Annexin V (+), PI (+) fraction
go down the drain if the prep was made using a Ficoll separation?
2- Is the Annexin V (+), PI(+) fraction something that might be
unintentionally gated out if the light scatter gate is too tight ?

Peter Lopez
The Aaron Diamond AIDS Research Center
212.448.5188 (office)
212.448.5159 (fax)
212.448.5190 or 5110 (lab)

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