Re: Apoptosis, Annexin V and Ficoll

From: Ray Hicks (rh208@cam.ac.uk)
Date: Thu Mar 22 2001 - 12:29:16 EST

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Hi Peter,

Yes to both of these, the AxV/PI correlates well with lower FSC/higher SSC,

Ray

> From: PLopez@adarc.org
> Date: Wed, 21 Mar 2001 17:02:48 -0500
> To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
> Subject: Apoptosis, Annexin V and Ficoll
>
>
> Hello,
>
> I have two questions related to the Annexin V apoptosis thread:
>
> 1- In human PBMC preps, would the Annexin V (+), PI (+) fraction
> go down the drain if the prep was made using a Ficoll separation?
> 2- Is the Annexin V (+), PI(+) fraction something that might be
> unintentionally gated out if the light scatter gate is too tight ?
>
> Peter Lopez
> The Aaron Diamond AIDS Research Center
> 212.448.5188 (office)
> 212.448.5159 (fax)
> 212.448.5190 or 5110 (lab)
>
>

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