DAPI as dead cell discriminator

From: David_C_McFarland@sbphrd.com
Date: Fri Mar 16 2001 - 09:38:02 EST

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I'm a little puzzled by the discussions about using DAPI as a dead cell
discriminating dye.  I was under the impression that DAPI passes intact
membranes.  In fact, Molecular Probes technical support confirmed that DAPI is
not a suitable substitute for PI, 7AAD, etc as a dead cell discriminator.  What
gives?


David McFarland
GlaxoSmithKline

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