Dear group, I had an unusually large batch of monocyte cytokine stains to do yesterday, and the acquisition took a lot longer than usually. Long enough, in fact, to notice that between the control sample (acquired at time=0) and the last patient (acquired at time=3.5 hrs) CD33 FITC and CD45PE-Cy5.5 signal went up by half a log. The samples were fixed and washed multiple times so there's no way there was any free antibody left to "add" more signal. Why did the signal go up so much? The unstained, external and internal isotype stains were unaffected. Any thoughts would be appreciated, Maciej __________________________________________________ Do You Yahoo!? Yahoo! Auctions - Buy the things you want at great prices. http://auctions.yahoo.com/
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