YFP

From: Jacqueline.Saleh@aventis.com
Date: Tue Mar 13 2001 - 10:23:42 EST

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Dear Flowers:

I have an investigator here who is doing cotransfection using YFP/RFP. Under
the
microscope he can clearly see the positive RFP cells. However maximal
excitation
for RFP is about 585 so I 'm getting a very weak signal in the FL2 channel.
Also
the YFP signal is so bright it tails into the double positive quadrant. What
is the
best combination of fluorescent proteins to use to get optimal separation?
Thanks in advance for your help.

Jackie Saleh
Aventis Pharmaceuticals
jacqueline.saleh@aventis.com

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