I have used BioWhittaker's X-VIVO 15 with good results. Cheers Lesley Mrs. Lesley Barber Cell Manipulation Scientist Edith Margaret Collie Centre for Blood Cell Therapies Division of Haematology and Medical Oncology Peter MacCallum Cancer Institute St Andrews Place, East Melbourne, VICTORIA , 3002, AUSTRALIA. Tel +61 3 9656 1957 or: Page (via switch) +61 3 9656 1111 (pager 1271) Fax +61 3 9656 1811 Mobile: 0428 977 278 > -----Original Message----- > From: Heather Medbury [SMTP:Heather_Medbury@wsahs.nsw.gov.au] > Sent: Monday, March 12, 2001 9:50 AM > To: Cytometry Mailing List > Subject: Hi FLOWers, > > > Hi FLOWers, > > In your opinion what is the best serum free media to use for culturing > lymphocytes > in. I will be doing intracellular cytokine staining of antigen stimulated > lymphocytes > but want to avoid using serum. > > Any comments on intracellular cytokine staining methods is also welcome. > > Heather > > > Heather Medbury (PhD) > Department of Surgery > Westmead Hospital > Westmead 2145 > 61-2 9845 7677 > -------------------------------------------------------------- > All experiments are preliminary > > ------------------------o0)(0o-------------------------------------- > > "In his heart a man plans his course > but the Lord determines his steps" > > ---------------------------------------------------------------
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