Hi FLOWers, About 5 years ago when I was analysing lymphocytes, we used to have the cell preparations at 4°C for labelling with the antibody. The work I do now on whole blood, the cells are labelled at room temperature. Why do people label at 4°C when it works perfectly well at room temp? Heather Heather Medbury (PhD) Department of Surgery Westmead Hospital Westmead 2145 61-2 9845 7677 -------------------------------------------------------------- All experiments are preliminary ------------------------o0)(0o-------------------------------------- "In his heart a man plans his course but the Lord determines his steps" ---------------------------------------------------------------
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