I'm thinking of trying DAPI in the UV channel for live/dead gating instead of using 7-AAD in FL3, in order to free FL3 for three color gating and sorting strategies, on our FACS Vantage. My question is: Is there any spectral overlap/interference with the standard FL1, FL2 or FL3, ie. FITC, PE and PE-Cy5 or any other considerations I should be thinking of to do live/dead gating with DAPI? Your assistance, as always, is appreciated.
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