I have been using a different compound, Hydroxystilbamidine methanosulfonate (Fluoro-Gold) from Molecular Probes, for viability staining using the UV laser. My work was published in Cytometry in 1999 and the research labs at Peter MacCallum Cancer Institute have been using this compound for more than two years with good results. Cells sorted using the viable gate from this procedure have been grown in tissue culture and also retransplanted with no adverse results. See the article: Fluoro-Gold: An alternative Viability Stain for Multicolor Flow Cytometric Analysis. Lesley Barber et al, Cytometry 36:349-354 (1999) Cheers Lesley Mrs. Lesley Barber Cell Manipulation Scientist Edith Margaret Collie Centre for Blood Cell Therapies Division of Haematology and Medical Oncology Peter MacCallum Cancer Institute St Andrews Place, East Melbourne, VICTORIA , 3002, AUSTRALIA. Tel +61 3 9656 1957 or: Page (via switch) +61 3 9656 1111 (pager 1271) Fax +61 3 9656 1811 Mobile: 0428 977 278 > -----Original Message----- > From: MSchwa3722@AOL.COM [SMTP:MSchwa3722@AOL.COM] > Sent: Monday, March 05, 2001 10:37 AM > To: Cytometry Mailing List > Subject: viability gating > > I'm thinking of trying DAPI in the UV channel for live/dead gating instead > of > using 7-AAD in FL3, in order to free FL3 for three color gating and > sorting > strategies, on our FACS Vantage. My question is: Is there any spectral > overlap/interference with the standard FL1, FL2 or FL3, ie. FITC, PE and > PE-Cy5 or any other considerations I should be thinking of to do live/dead > > gating with DAPI? > > Your assistance, as always, is appreciated. >
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