Dear Paul, Although you didn't specify an application the answer to your question really depends on what you want to know. There have been lots of good discussions on this bulletin board about identifying and enumerating monocytes so I would first of all look back at those. I would caution against relying totally on CD14 for capturing all monocytes because we routinely see a population of otherwise normal monos that are CD14- (usually <5%). This lineage has been confirmed using additional markers such as HLA-DR, CD64, CD13, CD4 (weak), and bright CD33. Probably a simple method would be to observe the less bright CD45+ population vs log SS between the lymphs and grans which is just about as accurate as using CD14 positivity in our hands. There's a good article by Kransiskas, et al : "The Usefulness of CD64, Other Monocyte-Associated Antigens, and CD45 Gating in the Subclassification of Acute Myeloid Leukemias with Monocytic differentiation."; AM J CLIN PATHOL 1998; 110:797-805. Hope this helps, Bruce B.Greig, Immunopathology Vanderbilt Univ. Med. Center Nashville, TN. 37232 (615)322-682
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