Glutathione detection

From: Scott Tighe (stighe@zoo.uvm.edu)
Date: Fri Mar 02 2001 - 16:45:04 EST


Dear Flowers

We need to detect Glutathione in rat tumor cells and understand that
only several dyes exist from mostly Molecular Probes. I would like to
hear from anyone that has tried to do this on their cell line using
flow. I  have read that data collected using flow can be somewhat
ambiguous. Have you been successful? Which dyes can be used? Which work
best?  What are the drawbacks?

Scott Tighe
Vermont Cancer Center
Flow Cytometry Core Lab
802.656.1333



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