Re: Identification of SP cells

From: ckuszyns@UNMC.EDU
Date: Thu Mar 01 2001 - 16:40:31 EST

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60mw is plenty to excite the dye and define a Hoechst low population.  I am
currently using an Enterprise laser at 50mW to sort SP cells at speeds of
5-6000 per second.  You may wish to look at the dye concentration and time
of loading as parameters .

Charles A. Kuszynski, Ph.D.
Assistant Professor/Director
Cell Analysis Facility
University of Nebraska Medical Center
986495 Nebraska Medical Center
Omaha, NE 68198-6495
402 559-6267 voice
402 559-4077 fax
ckuszyns@unmc.edu

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Maybe in reply to: Martin White: "Identification of SP cells"
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