Hi Flowers, I wish to identify SP cells distinguished via their low fluorescence in the blue (using a 450/20 BP filter) and the red (using a 675EFLP optical filter) following excitation of Hoechst dye with a uv laser line. Currently I'm using a Coherent Innova Enterprise with dual visible and uv lines, however the uv power output (<60mwatts) is insufficient to optimally excite the dye.I would be very grateful for anyone with SP experience who could advise me as to their laser configuration to achieve higher uv laser output In addition, I run a FACSVantage SE and would like to hear from anyone using this sorter to identify SP cells what optical / PMT configuration they use for emission detection. Thanks! Martin White, London, Ontario. Martin White, London, Ontario, Canada
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