At 1:53 PM +0100 21/2/01, Kerstin wrote: >Hello everybody, >does anybody know a protocoll/paper for isolating DC of Mouse, label them >with CFSE, bring them back in a mouse and search later for their >localization? >Thanks Kerstin > We have done this with CMFDA and it was published in JExMed in 1999. The reference is Smith, A. L. and B. Fazekas de St. Groth (1999). "Antigen-pulsed CD8a+ Dendritic Cells Generate an Immune Response after Subcutaneous Injection without Homing to the Draining Lymph Nodes." Journal of Experimental Medicine 189(3): 593-598. I have done it with CFSE but it can be tricky because CFSE can be quite toxic to the cells than CMFDA so you have to buffer the labelling solution with extra protein (the paper is only a Brief Definitive Report so the methods are fairly abbreviated). Feel free to ask any specific questions. Hope that helps, Adrian -- ****************************************************** Adrian Smith T CELL BIOLOGY GROUP Centenary Institute of Cancer Medicine & Cell Biology Locked Bag No.6 Newtown, NSW 2042 AUSTRALIA. Ph: (02) 9565-6198 Fax: (02)-9565-6103 ******************************************************
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