Hello, I am posting this from a colleague who is looking for a protocol for thawing and disaggregating snap frozen tissue for cell sorting for expression arrays. Any experience or advice would be appreciated. Thanks, -Bill From: Annette Byrne [mailto:byrnea@obgyn.ucsf.edu] Sent: Friday, February 23, 2001 4:55 PM To: cyto-inbox Dear Bill Hope you are well. I am writing to you about the feasibility of using snap frozen tissue for separation of cellular subpopulations by flow using surface and viability markers. Further to this, I was wondering whether you could recommend/ supply a protocol for this purpose. Having spoken with various bodies, there appears to be some controversy whether cells remain intact adequately after snap freezing for use with flow cytometry. What do you think ? Many thanks for you help Regards Annette Byrne Annette Byrne PhD Research Fellow University of California San Francisco Dept of Obstetrics, Gynaecology & Reproductive Sciences Box 1656 HSW CRS-OB/GYN 505 Parnassus Ave. San Francisco CA 94143-0556 USA e mail : byrnea@obgyn.ucsf.edu Tel: 001-415-5025173 Fax: 001-415-5027866
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