At a local flow cytometry users group ( RTCA) on Feb 22 2001, we heard a about the BD DIVA digital electronics. It was impressive and like all new developments in flow cytometry it is a welcomed addition. BD is now measuring area on all pulses instead of height that is normally used on previously versions of BD equipment. For you BD users, there is one board in the machines currently that can yield area measurements which are required for DNA measurements using Doublet discrimination. Now I remember that our old Ortho 50H yielded area measurements on all signals and this is the way we always ran our machine. We reluctantly gave up this measurement to use the User friendly BD FacsCalibur with their CellQuest program. My questions are the following: How is our Flow data going to change by using area instead of peak height measurements It should be more accurate with area but how bad is the height measurement that we have obtained for many many years.. This Question was raised at the meeting but it was not adequately answered by anyone in the audience. How bad is height measurements?. Is it only bad ( inaccurate) for linear measurements like DNA??? Let's discuss this. I think it is important. Bob Robert M. Zucker, PhD U.S. Environmental Protection Agency MD 72 National Health and Environmental Effects Research Laboratory Research Triangle Park, North Carolina, 27711 Tel: 919-541-1585; fax 919-541-4017 e-mail: zucker.robert@epa.gov
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