After Howard wrote me that he hadn't said what I thought he said I have to apologise. I was to dense to read "density of fluorescent.." and read "intensity" instead. I was actually trying to think about the slit scanning peak to reflect the intensity distribution within the cell. In case of the ideal doublet you get a 'twin peak' signal but still the correct area or integral for the total event. Thus the peak in slit scans does not reflect total fluorescence but the maximal signal density as said by Howard in the first place. I think I have to get a faster / digitised internal translation chip (or a coffee). Gerhard by the way thanks for the pun Howard Howard wrote: When the illuminating beam height is close to or smaller than the cell or particle diameter, passage through the beam produces a "slit-scan"; the pulse height is now proportional to the density of fluorescent or scattering material in the cell, but not to the area. The height is thus sensitive to shape; the area is not, and that is why plots of peak vs. integral can be used to remove data from many (but usually not all) doublets in DNA analysis in instruments with small beam heights.
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