Hoechst and BrdU

From: David Dombkowski (dombkows@helix.mgh.harvard.edu)
Date: Wed Feb 14 2001 - 11:21:12 EST

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 I am trying to use a pre-incubation with hoechst 33342 to stain DNA
followed by fixation and permeabilizing with the BD Pharmingen Brdu flow
kit. After treating cells with Dnase and staining for Brdu I notice a high
background in fitc channel with BrdU-fitc staining. I would like to know if
this has something to do with Hoechst staining followed by BrdU staining
and whether it would be better to use another UV DNA dye. I would
appreciate any comments on this issue. Thanks.

David M. Dombkowski
dombkowski@helix.mgh.harvard.edu
Flow Cytometry-Pathology-CNY rm7017
149 13th Street
Massachusetts General Hospital-East
Boston, MA 02129
Tel. (617)726-1683
Fax.(617)724-3164

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