Bill:
See Cytometry 29:286-291, 1997; "Use of a membrane-localized green fluorescent protein allows simultaneous identification of transfected cells and cell cycle analysis by flow cytometry"; Kalejta, et al.
Ok, so where do we collect on the beer!
Joanne
Joanne Thomas, M.S.
Director of Operations
TRITECH Field Engineering
2014 Renard Court, Suite I
Annapolis, MD 21401
1-800-886-7004 (USA)
1-410-266-1522
410-266-0935 (FAX)
thomas@tritechinc.com
-----Original Message-----
From: Hyun, Bill <hyun@cc.ucsf.edu>
To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
Date: Wednesday, February 14, 2001 5:18 PM
Subject: fixation for GFP flow
Hello,
I would greatly appreciate a fixation protocol or reference for running EGFP and measuring cell cycle with PI for flow. Any help will get a free beer from Julie Auger. Thanks.
-Bill
*****************************
William C. Hyun, Director
Laboratory for Cell Analysis, S129
UCSF Comprehensive Cancer Center
San Francisco, CA 94143-0808
phone: (415)476-2632
FAX: (415)476-8218
email: hyun@cc.ucsf.edu
Deliveries:
2340 Sutter Street Rm129
San Francisco, CA 94115
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