Liza, I'm no expert, but (or rather, because of that!) I attended the Annual Clinical Course in Flow Cytometry sponsored by the Clinical Cytometry Soc. last August at Northwest Univ. and one of the lecturers in particular addressed the topic of subpopulations of CD34+ cells and engraftment--Michael Keeney, at the London Health Sciences Centre in London, Ontario: mike.keeney@lhsc.on.ca It was an interesting lecture. There is a difference in how readily different subsets engraft, but, he said in most cases, measuring CD34 subsets is probably not clinically relevant and the number of viable CD34 cells actually infused is probably the most relevant parameter, clinically. He said measuring CD34+CD19+ may be useful in poor mobilisers with marginal collections. This is a fascinating topic, and I wish my notes were more complete. He did mention a study of a CD34+CD33- subset and engraftment which indicated this subset was the only significant predictor of RBC and platelet usage. (Pecora, A., et al). He also mentioned AC133 antigen (being clustered to CD133). Utilizing AC133 may prove to be more useful in enriching stem cells than CD34 alone. (Gallacher, L et al) See references below. Gallacher, Lisa, et al, Isolation and characterization of human CD34-Lin- and CD34+Lin- hematopoietic stem cells using cell surface markers AC133 and CD7, Blood, 1 May 2000, Vol. 95, No. 9, pp. 2813-2820. Pecora, A., et al, CD34+CD33- Cells Influence Days to Engraftment and Transfusion Requirements in Autologous Blood Stem-Cell Recipients, Jo. of Clin. Onc, Vol. 16, No. 6 (June), 1998, pp. 2093-2104. Weaver, A. et al, mobilization of long-term culture initiating cells with use of chemo, G-CSF and stem cell factor, Blood, 88:9 (Nov 1) 1996, 3323-3328. Hope you find these articles or the bibliographies helpful. I recommend the course highly. I see it is being offered in Montpellier, in la belle France in May. One will long for a nice vacation after this intense course. If you've never been to France, and don't have time to take extra days or weeks for enjoyment of the culture and MAGNIFICENTcuisine, don't try to combine the two. Betsy Caldwell
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