Marty, Molecular Probes has a kit for assessing cell cytotoxicity that is
based on one of this reagents and PI. We have used it and is ok. I have
submitted a proposal for a chapter in CPCy that include the protocols for
assessing in vitro and in vivo killing assays. I encourage you to try. The
only problem is that you should find the appropriated concentration for
your cells but a simple titration test will be fine. I hope this will help
you. Regards Rafael
>Does anyone have any experience using Molecular Probes DiD as a
>non-specific membrane marker for human lymphoid cells? If so, email
>me - I would like to talk.
>
>Marty Bigos
>Director
>The J. David Gladtone Institutes Flow Cytometry Core
>mbigos@gladstone.ucsf.edu
>(voice) 415-695-3832
>(fax) 415-826-8449
>
>Mailing Address:
>Gladstone Institutes
>P.O. Box 419100
>San Francisco, CA 94141-9100
>
>Shipping Address:
>Gladstone Institutes
>365 Vermont Street
>San Francisco, CA 94103
\|/
(o o)
________________________________oOo__(_)__oOo_________________________________
___/\_ | Rafael Nunez mailto:rafaeln@vetvir.unizh.ch
/ o \/| | University Inst.for Virology http://www.vetvir.unizh.ch/
/ _| | Winterthurerstr. 266a Telephone: (+41) 1 6358710
/_/\__/-\/ | 8057 Zurich SWITZERLAND Faximile : (+41) 1 6358911
______________________________________________________________________________
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