Lynn We routinely sort for cells that constitute 0.01% of the threshold events. We have done some where the frequency was 0.001%. The ability to resolve frequencies this low require good signals so that you have separation between the negatives and the positives. If the populations overlap when negatives and positives are in the same sample then the frequency you can resolve reliably becomes larger. Whoever you were arguing with was right and wrong depending on the circumstances. Larry At 01:39 PM 1/26/2001 -0500, you wrote: >Hello all, > >This question arises from a rather heated discussion following a seminar >yesterday, in which the speaker claimed that flow cytometry is not >useful for analyzing cells that are less than 1-2% of the starting >population. I am sure that with all of the sorting, multicolor analysis, >and multiparameter gating that people do, we can prove this assertion >wrong. > >If you have experience or publications with analysis of events well >below 1% of the starting cell population, could you please share some >examples or references? > >Thanks in advance for your help! >********************************************** >Lynn B. Dustin, Ph.D. >Center for the Study of Hepatitis C >Rockefeller University >Box 64 >1230 York Ave. >New York, New York 10021 >Phone: 212-327-7067 >email: dustinl@mail.rockefeller.edu Larry W. Arnold, Ph.D. Res. Assoc. Prof. Director, Flow Cytometry Facility Department of Microbiology and Immunology Lineberger Comprehensive Cancer Center CB# 7290 University of North Carolina at Chapel Hill Chapel Hill, NC 27599 Phone: 919-966-1530 FAX: 919-962-8103
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