Re: very rare events: how low can you go?

From: Simon Monard (smonard@trudeauinstitute.org)
Date: Tue Jan 30 2001 - 09:20:08 EST


If you look at almost ant tetramer publications you'll find people looking at populations
much less than 1%. Search for publications by G. Ogg.



Simon Monard
FACS Lab Manager
Trudeau Institute
Saranac Lake
NY12983

Ph 518 891 3080 X352


>>> Lynn Dustin <dustinl@mail.rockefeller.edu> - 1/26/2001 1:39 PM >>>

Hello all,

This question arises from a rather heated discussion following a seminar
yesterday, in which the speaker claimed that flow cytometry is not
useful for analyzing cells that are less than 1-2% of the starting
population. I am sure that with all of the sorting, multicolor analysis,
and multiparameter gating that people do, we can prove this assertion
wrong.

If you have experience or publications with analysis of events well
below 1% of the starting cell population, could you please share some
examples or references?

Thanks in advance for your help!
**********************************************
Lynn B. Dustin, Ph.D.
Center for the Study of Hepatitis C
Rockefeller University
Box 64
1230  York Ave.
New York, New York 10021
Phone: 212-327-7067
email: dustinl@mail.rockefeller.edu



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