Dears The most recently evidence is tetramer staining during viral infections. The frequency is below 1% of whole lymphocytes. Some conditions are necessary : the number of events analysed will be high (50000-500000) and the positive staining will be quite different from negative staining. In our laboratory we can go to detect 0.07% positive cells. You can found several publications with the keywords tetramer and HIV. Sincerely yours; -- Dr Daniel SCOTT-ALGARA Service d'Immuno-hématologie et d'Immunopathologie Institut Pasteur 75724 Paris Cedex 15 France Phone 33 (0)1 45 68 82 13 Fax 33 (0) 1 45 68 89 51 E-mail: scott@pasteur.fr
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