Hi Andrew, I just tried this for the first time last week. And beginners luck I guess, worked great. I was doing CFP/YFP and for the YFP excited with 488 and picked up with a 550/30 BP After the standard 560 SP dichroic into FL1. For CFP I excited with a 406nm Krypton line And picked up with a 485/22 BP past a 505SP into the FL4 channel on the Vantage. Separation of both signals from the double positive was excellent with little or no 488 Entering FL4 from the first laser - so I was thankful I didn't have to shift the first laser into The 512nm line and lose my scatter or use only one laser at 458 and lose scatter and have compensation issues. Of course I thank Santa for the Krypton laser. Gene/ http://flowcytometry.uchc.edu <http://flowcytometry.uchc.edu> -----Original Message----- From: Andrew Beernink [SMTP:ABeernink@novasite.com] Sent: Friday, December 08, 2000 12:04 PM To: Cytometry Mailing List Subject: cyan fluorescent protein... Hi- What are people using to excite Cyan Fluorescent Protein? We have a 457 line, but unfortunately it excites other dyes we're using. Anybody using a 440 HeCd, or even better, a 413 Krypton line? Thanks. Andrew Beernink Novasite Pharmaceuticals
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