Andrew, We've used the 413 line from our krypton in recent studies (Science Jun 30 2000: 2354-2357 and (www.stke.org/cgi/content/full/OC_sigtrans;2000/38/pl1); Roger Tsien suggested that the HeCad 440 line is optimal, but this was for microscopy. I'd like to see if this is true for flow as well, but I don't have a HeCad to try at the moment. Kevin Kevin L. Holmes, Ph.D. Head, Flow Cytometry Section Research Technologies Branch NIAID, NIH Bldg. 7, Room 01 NIAID, NIH Phone: 301-496-9071 FAX: 301-402-4532 Email: kholmes@niaid.nih.gov -----Original Message----- From: Andrew Beernink [mailto:ABeernink@novasite.com] Sent: Friday, December 08, 2000 12:04 PM To: cyto-inbox Subject: cyan fluorescent protein... Hi- What are people using to excite Cyan Fluorescent Protein? We have a 457 line, but unfortunately it excites other dyes we're using. Anybody using a 440 HeCd, or even better, a 413 Krypton line? Thanks. Andrew Beernink Novasite Pharmaceuticals
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