Kevin Homes wrote, in response to Andrew Beernink's query about excitation for cyan fluorescent protein - >We've used the 413 line from our krypton in recent studies (Science Jun 30 >2000: 2354-2357 and (www.stke.org/cgi/content/full/OC_sigtrans;2000/38/pl1); >Roger Tsien suggested that the HeCad 440 line is optimal, but this was for >microscopy. I'd like to see if this is true for flow as well, but I don't >have a HeCad to try at the moment. The excitation spectrum is what it is; if 440 nm is optimal for microscopy, it should be for flow as well. Note that most of the He-Cd lasers used in flow cytometers emit at 325 nm and do *not* have interchangeable mirrors, so you'd need a new 440 (closer to 442, actually) nm He-Cd laser. But you can get 100 mW of 442 nm excitation out of a He-Cd laser which is air-cooled and runs on 110 VAC, although it will cost you $20K or so. -Howard
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:41 EST