Re: Lysing RBC in different animal species

From: Carol Oxford (cloxford@UCDAVIS.EDU)
Date: Thu Nov 30 2000 - 07:56:00 EST


We've used Coulter's Q-prep for mice, canine, feline, and primate samples
without any problem.  We've also used FACS Lyse for primate and mouse
samples.

Carol

>Hello Flowers,
>
>I have had a request to analyze blood from a variety of species.  I will
>need to lyse the RBC's of these animals.  Those of you who have analyzed
>animal blood. What do you recommend as the best lysing method? I have
>experience with Ammonium Chloride , FACS Lyse and Coulter Lyse. The species
>I will be evaluating are: dog, rat, moue, rhesus, cyno, chimp, baboon,
>pig-tail macaque, marmoset, cotton-top tamarin, pig, and green monkey. Any
>recommendations or references would be greatly appreciated.
>
>Thanks,
>
>Sue
>
>Susan M. Schmitt
>Manager, Clinical Immunology
>IDEC Pharmaceuticals
>3010 Science park Road
>PO Box 919080
>San Diego, CA 92191-9080
>
>
>Ext. 8492


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Carol Oxford                             _|    _|   _|_|_|_|   _|_|_|
Manager, UC Davis Optical Biology Lab   _|    _|   _|         _|    _|
Tupper Hall, rm 3425		       _|    _|   _|	     _|    _|
University of California	      _|    _|	 _|	    _|	  _|
Davis, California  95616	     _|    _|	_|	   _|	 _|
(530) 752-7205			    _|	  _|   _|	  _|	_|
(530) 752-4548 fax		   _|_|_|_|   _|_|_|_|	 _|_|_|
(916) 523-0967 pager
cloxford@ucdavis.edu		   University of California, Davis
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