We (at Los Alamos National Laboratory) published a method for detection of antigen-stimulated lymphocyte subset proliferation, which combines BrDU incorporation, or PI or DAPI staining, with Immunophenotyping. "Detection of beryllium sensitivity using a flow cytometric lymphocyte proliferation test: The Immuno-Be-LPT" Toxicology, 143:125-140, 2000. >Hi all > >Not strictly a flow question, but I plan on using flow to examine the >cellular response > >I wanted to look at the human immune response to particular antigens. >Iwanted to add >antgien to human leukocytes in culture and detect the immune response by >flow and >proliferation assays. >I realise however that the immune response will involve a very small >number of T cells >specific for the antigens, so I probably wouldnt be able to detect the >response. Though >I would look by flow for the activated T cells and examine them only. > >Any hints, comments or suggestions on whether it will work or how I can >get it to work. >Any references you know of that may be useful > >Thanks > >Heather Medbury >Sydney Aus. Babetta L. Marrone, Ph.D. B-N2, M888 SM-30, Bikini Atoll Rd. Los Alamos National Laboratory Los Alamos, NM 87545 Voice: 505-667-3279 FAX:505-665-6894; 505-665-3024 email: blm@lanl.gov Home Page: http://fish.lanl.gov
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