For flow, CD69 is the first thing to try, and it's neat b/c it can be done on specific cell populations (ex. 19/69/45, 4/69/45, 56/69/45 etc...) The whole test including incubation, acquisition and analysis takes about 6 hours. Cytokines take a wile longer especially in Lymphocytes. These also can be divided into specific cell populations, as long as your activation doesn't induce shedding of CD's (as in.. don't use LPS and CD4 or 14) Long term activation and proliferation can be also assayed using Tritium labeled thymidine uptake. Regards, Maciej ===== `---------------------------------------------` | Maciej S. Simm | 525 E 68th Street | | Research Technician | Room N-805 | | Cornell Medical Center | Tel. 212.746.3428 | `---------------------------------------------` |www.cd4cd8.com | 917-734-6280 | AIM - XsimmX | `---------------------------------------------` __________________________________________________ Do You Yahoo!? Thousands of Stores. Millions of Products. All in one Place. http://shopping.yahoo.com/
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