In reply to Scott Tighe's question on yeast cell cycle: I would like to point out that we recently published a unit in CPC on yeast cell cycle: Unit 11.13 F. Sansonetty et al. Cell Cycle Analysis in Yeasts Using SYBR Green I you can find Current Protocols in Cytometry at http://www.wiley.com/cp/cpc/ Paul Robinson Editor, Current Protocols in Cytometry --------------- On 1 Nov 2000, at 11:37, Scott Tighe wrote: Greetings fellow flowers Can anybody recommend the best way to model the cell cycle for budding yeasts? I currently use ModFit 2.0 for our cancer studies, but it does not lead itself to budding yeasts. I am normally working with mammalian cells and do not have this trouble. Is there a standard method to set-up these experiments when ultimately one will need to model the yeast's cell cycle with curve-fitting software? Sincerely Scott Tighe Vermont Cancer Center University of Vermont 214A Medical Alumni Bd. Burlington, VT 05405 J.Paul Robinson, PhD PH:(765)4940757 Professor of Immunopharmacology Professor of Biomedical Engineering Purdue University FAX:(765)4940517 EMAIL:jpr@flowcyt.cyto.purdue.edu WEB: http://www.cyto.purdue.edu
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