Dear Flow'ers, We are facing difficulties in alignment of the FACS Vantage SE and this question regards the availability of concensus protocols and papers that describe the caliberation of the machine. We use Calibrite beads regularly for caliberation. But we are not able to get the same reading for the peak channels for FL1,2,or 3, without changing the voltages. Would it not be a better idea to use sphero rainbow particles (even if they are expensive). The manual is somehow not adequate enough for trouble shooting this. I would be grateful for any help regarding this. Can some one also give some figures as to what readings they obtain upon caliberation with calibrite beads, and any variations in their alignment protocol. Thanks Regards Rana ____________________________________________________________________ Get free email and a permanent address at http://www.netaddress.com/?N=1
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