yeast cell cycle

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Dear Flowers

I have recently started doing cell cycle analysis on the budding yeast,
Saccharomyces cerevisae. I have used the protocol of Fortuna et. al. in
Current Protocols in Cytometry. This method uses SYBR green I and
produces a very nice cell cycle distribution. However, interpreting it
is somewhat different then mammalian cells which I am used to dealing
with. The cells exhibit budding (up to two per mother cell). I have
attached a picture of my histogram and was hoping someone experienced in
yeast cell cycle could comment/identify these populations. Also, Are any
of you aware of a compound that can be used to block yeasts in G2?

Scott Tighe
Flow Cytometry Core
Vermont Cancer Center
Burlington, VT.

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