> ---------- > From: Maciej Simm > Sent: Tuesday, 31 October 2000 1:17 > To: Cytometry Mailing List > Subject: in case of isotype control - substitute? > > Maciej, > > I dont bother with isotype controls for IL-10 production by > monocytes, I find unstimulated monocytes do not make any IL-10 and so use > this as my "negative" control. > > Greg Hodge PhD > hodgeg@mail.wch.sa.gov.au > -------------------------------------------------------------------------- > ---------------------------------------------- > > On 31 October, Maciej wrote- > > Howdy Everyone, > > I am running IL-10 in human monocytes using a PE conjugated Rat anti > Human IgG1 antibody. There is a shortage of intracellular isotype > control for that. What can I use instead? someone recommended using > anti rat IgG2 etc.. but I've read on here earlier the 2's are picked > up by monocytes.. > > so how about a mouse IgG1? > > Maciej > > __________________________________________________ > Do You Yahoo!? > Yahoo! Messenger - Talk while you surf! It's FREE. > http://im.yahoo.com/ >
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