in case of isotype control - substitute?

From: Maciej Simm (simmmmer@yahoo.com)
Date: Mon Oct 30 2000 - 09:47:28 EST

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Howdy Everyone,

I am running IL-10 in human monocytes using a PE conjugated Rat anti
Human IgG1 antibody. There is a shortage of intracellular isotype
control for that. What can I use instead? someone recommended using
anti rat IgG2 etc.. but I've read on here earlier the 2's are picked
up by monocytes..

so how about a mouse IgG1?

Maciej

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Next message: Abdullah Alasri: "p-53 neg and pos cells"
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Next in thread: Hodge, Greg (HAEM): "RE: in case of isotype control - substitute?"
Maybe reply: Hodge, Greg (HAEM): "RE: in case of isotype control - substitute?"
Maybe reply: Calman Prussin: "RE: in case of isotype control - substitute?"
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