I would agree with Greg. If you have a real negative control you don't need an isotype. Often isotypes are not true negatives, that is, they don't mimic the non-specific binding of the antibody, even tho' they are isotypically matched. When you have a negative population you can use, that is better than an isotype. Candace Enockson Medical University of South Carolina --On Tue, Oct 31, 2000 7:49 AM +1030 "Hodge, Greg (HAEM)" <hodgeg@mail.wch.sa.gov.au> wrote: > > > >> ---------- >> From: Maciej Simm >> Sent: Tuesday, 31 October 2000 1:17 >> To: Cytometry Mailing List >> Subject: in case of isotype control - substitute? >> >> Maciej, >> >> I dont bother with isotype controls for IL-10 production by >> monocytes, I find unstimulated monocytes do not make any IL-10 and so use >> this as my "negative" control. >> >> Greg Hodge PhD >> hodgeg@mail.wch.sa.gov.au >> ------------------------------------------------------------------------ >> -- ---------------------------------------------- >> >> On 31 October, Maciej wrote- >> >> Howdy Everyone, >> >> I am running IL-10 in human monocytes using a PE conjugated Rat anti >> Human IgG1 antibody. There is a shortage of intracellular isotype >> control for that. What can I use instead? someone recommended using >> anti rat IgG2 etc.. but I've read on here earlier the 2's are picked >> up by monocytes.. >> >> so how about a mouse IgG1? >> >> Maciej >> >> __________________________________________________ >> Do You Yahoo!? >> Yahoo! Messenger - Talk while you surf! It's FREE. >> http://im.yahoo.com/ >>
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:37 EST