Simon, We have used R123 to sort cells per their p-Gp status for years. You can use it with surface markers rather effectively. We've also used R123 and surface labels in combination with indo1 to measure subset calcium level vs p-Gp status. (Witkowski JM and Miller RA. Calcium signal abnormalities in murine T lymphocytes that express the multidrug transporter P-glycoprotein. Mechanisms of Ageing and Development 170:165-180, 1999.) MAK. Simon Monard wrote: > Hi > > We are interested in identifying and sorting p-glycoprotein bearing cells. We thought > that using a dye like rhodamine123 or Hoechst then sorting unlabelled cells would be > OK, any thoughts? What are the dyes of choice for this sort of thing? I hear that the > antibodies to g-gp aren't that good. It would make life easier if it were a 488 excited > dye although UV or 633 would be OK. We want to use several phenotypic markers as well. > > Thanks for any help > > Simon Monard > > Simon Monard > FACS Lab Manager > Trudeau Institute > Saranac Lake > NY12983 > > Ph 518 891 3080 X352 -- Mark A. KuKuruga, Managing Director University of Michigan Core Flow Cytometry <http://www.cancer.med.umich.edu/flow_cytometry> phone: 734-647-3216 fax: 734-936-7376 kukuru@umich.edu
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