Hi Simon, I have done a lot with R123 in a previous phase and it works quite well. The emission is relatively broad so that analysis of multiple phenotypic markers may be a challenge. The signal:noise is excellent but I would suggest always having the untransfected parentals to use as a control. There are a good variety of p-gp expressing cell lines around to use for assay set-up as well. -Jim Weaver ************************************************* * * * James L. Weaver Ph.D. * * Division of Applied Pharmacology Research * * Office of Testing & Research * * CDER MOD-1, FDA * * 8301 Muirkirk Rd, Laurel MD 20708 * * * * Phone: 301-827-8237 * * Fax: 301-594-3037 * * Email:WEAVER@CDER.FDA.GOV * * * *************************************************
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