We occasionally see really weird findings like this, although we haven't seen CD8 and CD19 coexpressed. What we do in a situation like this is re analyze the specimen, or in the case of peripheral blood get another specimen. We would look at the antibody in question in FITC, PE, and a third color if possible and use 2 different clones ( for example 2 different sources). We would also pair it with something else- like CD20 if CD19 was used last time. We would also make sure the blood was washed several times before staining as artifactual staining can be observed in unwashed blood ( Commun. Clin. Cytomet. 18:140-146, 1994). If it is still positive under all of these conditions then we would consider it real. Until then- it could be artifact. Maryalice >Has anyone seen a case that looks like classic CLL (dim kappa+, CD5+, dim >CD20+, CD23+, CD43+, CD10neg, CD3-,CD2-, CD7-, CD4-) BUT with CD8positivity >that coexpresses CD19? What do you think? >thanks, bessie >-- >Bessie Kam Email: bkam@dls.queens.org >The Queen's Medical Center, Pathology FAX: 808-547-4045 > Phone: 808-547-4271 Maryalice Stetler-Stevenson Director Flow Cytometry Unit Laboratory of Pathology, NCI, NIH
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