RE: Cell Volume Revisited

From: Robert C. Leif, Ph.D. (rleif@rleif.com)
Date: Sat Oct 14 2000 - 00:01:57 EST


From: Bob Leif
To: cyto-inbox

Unfortunately, I do not know of any commercially available focused flow
Coulter impedance analyzers except those in Beckman-Coulter clinical
hematology instruments.

-----Original Message-----
From: Andreas Simm [mailto:simm@klin-biochem.uni-wuerzburg.de]
Sent: Friday, October 13, 2000 1:06 AM
To: cyto-inbox
Subject: Re: Cell Volume Revisited



Hello Kevyn,
I know a second company, selling coulter like cell counters. The
people from this company always tell that their machine is better
than the original Coulter Counter ( as it is produced only for
analysing cells and not for industrial particle analysis as the
Coulter machine). As far as I know, it is sold in USA as well.

I know only the original address in Germany:

Schaerfe System
Tel.:	+49 - (0)7121 - 387-860
FAX:	+49 - (0)7121 - 387-8699

The name of this machine is CASY, the best upgrade is CASY-TTC.

The machine works fine, I used it for cells like fibroblasts,
lymphocytes, thrombocytes and cardiac myocytes.

Hope this helps

Andreas

>
> Flowers,
>
> Previously I had asked for help in determining the cell volume using a
flow
> cytometer.  I did not mention that a flow cytometer needs to be used and
> not a coulter counter because I am working with chicken cells.  I have
> tried coulter counters and they do not work on the avian blood I have
> tested.  Thus I need a different method.  It is believed that the
nucleated
> RBC of the chicken confuses the system.
>
> Thus a method on a flow cytometer is requested or a suggestion on getting
a
> coulter counter to work.
>
>
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> Thank You.
>
> Let's talk about MACS! - Visit our Forum at
> http://www.miltenyibiotec.com/index.php?site=forum
>
> Kevyn Adler, Ph.D.
> Technical Marketing Scientist
> Miltenyi Biotec
> 12740 Earhart Ave.
> Auburn, California 95602
> kadler@miltenyibiotec.com
> www.miltenyibiotec.com
> (800) 367-6227-Phone
> (530) 888-8871-Phone
> (530) 888-8925-Fax
>
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My address:

PD Dr. Andreas Simm
Institut fuer Klinische Biochemie und Pathobiochemie
Versbacher Str. 5
D-97078 Wuerzburg
Germany

Tel.:	+49 931-201-3144
FAX.: +49 931-201-3153
E-mail: simm@klin-biochem.uni-wuerzburg.de



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