We have documented the same phenomenon with other cell surface molecules (see J. Histochem. Cytochem. 44: 629-640 (1996)). In our experience this is quite general. The relevance in routine flow cytometry is high if you deal with highly-expressing shedder, and are looking for a dimly expressing cell population. Saverio Alberti Head, Lab. of Experimental Oncology Department of Cell Biology and Oncology Consorzio Mario Negri Sud 66030 Santa Maria Imbaro (Chieti), Italy Phone: (39-0872) 570.293 FAX: (39-0872) 570.412 E-mail: alberti@cmns.mnegri.it On Fri, 11 Aug 2000, Donald E. Mosier wrote: > > Colleaques- > > A recent paper by M. Mack et al. (Nature Medicine 6:769, July 2000) > documents transfer of CCR5 from positive to negative cells by > membrane-derived microparticles. Other surface proteins, particularly > 7-transmembrane spanners like chemokine receptors, may share this property. > Transfer is relatively efficient and rapid (e.g. 1 hr.). We have observed > similar effects in our experiments with cells transfected to express high > levels of CCR5, which then quickly begins to appear on negative cells in > the same culture. > > This is both a warning to be alert for this phenomenon in your experiments > and a request for more information on other cell surface molecules which > can translocate to negative cells. > > Don Mosier > __________________________________ > Donald E. Mosier, Ph.D., M.D. > Department of Immunology-IMM7 > The Scripps Research Institute > 10550 North Torrey Pines Road > La Jolla, CA 92037, USA > > ph 858 784-9121 > fax 858 784-9190 > > NOTE AREA CODE CHANGE! > >
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